Recombinase Aided Amplification Technology

RAA technology, short for Recombinase Aided Amplification, is a technology that utilizes recombinase, single-stranded binding protein and DNA polymerase to perform nucleic acid amplification under isothermal conditions (the optimal temperature is 39°C).

The specific principle is: Recombinase, single-stranded binding protein, and primer form polymers to scan double-stranded DNA, unwind double-stranded DNA at sequences homologous to the primers, and single-stranded binding proteins (SSBS) bind to the untwisted single strand to prevent single-stranded DNA refolding. In the presence of energy and dNTP, DNA polymerase completes the strand extension and repeats the process. The amplification can be detected by the instrument within 5-15 minutes.